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Created by sebastien.popoff on 27/06/2023
Dynamic structured illumination for confocal microscopy
Structured illumination enhances the resolution of a standard microscope by encoding the high spatial frequencies of an object's image into lower spatial frequencies through the use of a carefully selected pattern. In essence, it modifies the optical transfer function (OTF), which is the Fourier Transform of the point spread function (PSF), to increase sensitivity to high spatial frequencies. In [G. Noetinger et al, Arxiv 2306.14631 (2023)], the authors introduce a novel technique that further leverages time by incorporating a temporal periodic modulation, specifically through the use of a rotating mask, to encode multiple transfer functions within the temporal domain. This methodology is exemplified using a confocal microscope setup. At each scanning position, a temporal periodic signal is captured, enabling the construction of multiple images of the same object. The image carried by each harmonic is a convolution of the object with a phase vortex of topological charge, similar to the outcome when using a vortex phase plate as an illumination. This enables the collection of chosen high spatial frequencies from the sample, thereby enhancing the spatial resolution of the confocal microscope. |
